A process for production of a substrate for attachment of cells at certain positions and a substrate for attachment of cells. A support structure comprising a hydrophobic resin base material and a hydrophilic polymer layer formed on the hydrophobic resin base material. Areas of the hydrophilic polymer layer and a chemical reaction product present on a boundary surface between the hydrophilic polymer layer and the hydrophobic resin base material are removed, so as to expose areas of the hydrophobic resin base material at which cells will attach, by irradiating the support structure with UV radiation emitted from a light source via a pattern mask. The exposed areas are depressions with a controlled shape matched to the mask pattern, each of the depressions having a bottom surface which is formed by exposure of the hydrophobic resin base material.