The cold-adapted master strain A/Ann Arbor/6/60 7PI (H2N2) and progenitor
wild type E2(3) viral strains have been deposited and their genomic
sequences identified. Seven nucleotide differences were found between the
sequences identified herein and the previously published sequences for
cold-adapted A/Ann Arbor/6/60 genes. The cold-adapted live influenza
virus of the present invention can be reassorted with a variety of
epidemic wild type influenza viruses and used to produce vaccines to
prophylactically and therapeutically treat influenza.