Compositions for coating biological and non-biological surfaces, which
minimize or prevent cell-cell contact and tissue adhesion, and methods of
preparation and use thereof, are disclosed. Embodiments include
polyethylene glycol/polylysine (PEG/PLL) block or comb-type copolymers
with high molecular weight PLL (greater than 1000, more preferably
greater than 100,000); PEG/PLL copolymers in which the PLL is a dendrimer
which is attached to one end of the PEG; and multilayer compositions
including alternating layers of polycationic and polyanionic materials.
The multi-layer polymeric material is formed by the ionic interactions of
a polycation and a polyanion. The molecular weights of the individual
materials are selected such that the PEG portion of the copolymer
inhibits cellular interactions, and the PLL portion adheres well to
tissues. The compositions and methods are useful, for example, in
inhibiting formation of post-surgical adhesions, protecting damaged blood
vessels from thrombosis and restenosis, and decreasing the extent of
metastasis of attachment-dependent tumor cells. The compositions and
methods are also useful for coating non-biological surfaces such as
metallic surfaces.