A laser scanning microscope enables an observation of a specimen through
emitting a laser beam onto the specimen marked by a plurality of
fluorescent probes and receiving a fluorescent light back from the
specimen corresponding to the emission, and comprises a laser source for
generating a laser beam in an excitation wavelength corresponding to the
plurality of fluorescent probes, a deflector unit for scanning the
generated laser beam over an observation plane of the specimen, a
dispersion unit for dispersing a fluorescent light from the specimen to
extract it by an arbitrary wavelength interval, a spectral data
acquisition condition setting unit for setting a condition for the
dispersion unit acquiring a spectral data based on spectrum
characteristics of the plurality of fluorescent probes, a dispersion
control unit for controlling the dispersion unit based on the set
spectral data acquisition condition.