An inactivation effect of GSE on Norovirus is revealed and a sterilization solution suitable for inactivating Norovirus is provided. The sterilization solution has an effect of inactivating Calicivirus, and is prepared by dissolving a grapefruit seed extract (hereafter abbreviated as GSE) into water, adding phytic acid, and adding brewer's alcohol. In the sterilization solution, GSE is 0.08 percent by weight, phytic acid is 0.05 percent by weight, brewer's alcohol is 58.8 percent by weight, and water is the remainder. The pH is adjusted to become 5.8 to 6.4. A method for manufacturing a sterilization solution having an effect of inactivating Calicivirus includes the steps of dissolving GSE into water, adding phytic acid so as to emulsify, and adding 95-degree brewer's alcohol.