A hydrophobic grafted collagen, a method for preparing same and use thereof, in particular in therapy, are provided. Hydrophobic substances or molecules are grafted by covalent bonds on reactive amino acid residues of collagen molecules. The chemical linkages serve to modify the physico-chemical and biological properties of collagen and/or its derivatives. In particular, the introduction of hydrophobic residues enable the hydrophilic character of collagen to be modulated and its chemotactic properties involved in cell adhesion and growth to be modified.