It is intended to provide a chimeric animal producing a protein or tissue of an animal of the same or a different species or comprising a desired pluripotent cell transmitted to the germ line and to provide a pluripotent cell having a deletion of a particular gene and lacking a function involving the gene. The present invention provides a method for producing a chimeric animal, comprising injecting into a host embryo of an animal, two or more kinds of cells comprising a pluripotent cell having a mutation or deletion of a particular gene and lacking a function involving the gene and an additional pluripotent cell other than the pluripotent cell, and a chimeric animal produced by the method.