An ink-jet recording method and an ink-jet recording apparatus, wherein ink image with high quality and good abrasion resistance without generation of beading and bleeding are formed on recording media, in ink-jet recording using an intermediate transfer body, are provided. An ink image is formed by applying an image fixing component (for example reacting liquid) to an intermediate transfer body, and then applying inks of colors, Y, M, C, and K, from print heads corresponding each color. Then a supplementary liquid is applied to the ink image, and the ink image is transferred to a recording media.

 
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> Tango 240 polypeptides and uses thereof

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