The invention makes it possible to measure a calorie by using near-infrared rays, thereby realizing calorie measurement of an object to be quickly and easily performed using a non-destructive method. A device of measuring calorie of an object includes an object holding unit (1) including a table (2) on which an inspection-target object (M) is placed; a light source unit (20) that supplies light in near-infrared regions to irradiate the inspection-target object (M) placed on the turning table (2); a light reception unit (30) that receives light reflected from or transmitted though the object (M); and a control unit (40) that calculates the calorie of the object (M) in accordance with the absorbances of the light received by the light reception unit (30). In the control unit (40), the calorie of object (M) is calculated in accordance with a regression expression and the absorbances of the light received by the light reception unit (30). In this case, the regression expression is preliminarily calculated in the manner that near-infrared rays are irradiated on a calorie known sample object (M), and multiple-regression analyses are performed on second derivative spectra at the absorbances of light reflected from or transmitted though the sample object (M).

 
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> CIRCULAR PROBE AMPLIFICATION (CPA) USING ENERGY-TRANSFER PRIMERS

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