A peristaltic pump tubing system for use with a peristaltic pump roller assembly in a surgical procedure. The system comprises a uniquely shaped disposable cassette for retaining tubing to be used during the procedure and a uniquely shaped cassette receiving station situated adjacent the roller assembly. The cassette comprises a frame for holding the tube in a loop shape for engagement with the peristaltic rollers. The frame supports a one-sided pressure transducer to indicate the pressure within the tube. The cassette receiving station includes a sensor for engaging the one-sided pressure transducer and is situated adjacent the rollers of the peristaltic pump on the pump housing. A retaining bracket cooperates with the inherent resiliency of the tubing loop to hold the tubing in place against the rollers.