The object of the present invention is to provide a yeast which is tolerant to copper and which can incorporate copper at a high concentration, and also a method of removing or recovering copper from extracellular solution. The present invention is copper-tolerant yeast and the pectinases produced by the yeast. Particularly, the present invention is copper-tolerant yeast Cryptococcus sp. N6 strain isolated from deep-sea sediments and the pectinases produced by the yeast.