An image forming apparatus includes an image bearing body and a developing section. An electrostatic latent image is formed on the image bearing body. The developing section includes a developing member and a toner supplying member. The developing member is in contact with the image bearing body and applies toner to the electrostatic latent image. The toner supplying member is in contact with the developing member and has a peripheral surface that moves relative to the developing member. The toner has substantially spherical particles and is supplied from above the developing section and has a saturated apparent density not more than 0.4217 g/ml. The amount of the toner deposited on the developing member is in the range of 0.5 to 1.0 mg/cm2. The layer of the toner deposited on the developing member has a surface potential in the range of -50 to -250 V.

 
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> Method for in vitro molecular evolution of protein function using varied exonuclease digestion in two polynucleotide populations

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