An ophthalmic surgical cassette for removably receiving in a cassette receiving mechanism of an ophthalmic surgical system. The system includes first and second plunger valves. The cassette includes a body having a rear surface, an irrigation inlet for receiving irrigation fluid from a source, a first irrigation outlet for providing irrigation fluid to a first ophthalmic microsurgical instrument for use in an anterior segment ophthalmic surgical procedure, a first manifold fluidly coupling the irrigation inlet with the first irrigation outlet, a second irrigation outlet for providing irrigation fluid to a second ophthalmic microsurgical instrument for use in a posterior segment ophthalmic surgical procedure, and a second manifold fluidly coupling the irrigation inlet with the second irrigation outlet. The surgical cassette greatly simplifies a combined anterior segment and posterior segment ophthalmic surgical procedure by eliminating the need for separate anterior segment and posterior segment cassettes for the combined procedure.