Method for disrupting S. Cerevisiae yeast cells in an aqueous solution, by means of a continuous process under high pressure (up to 4000 bar), using a homogenizer.The suspension to be processed passes through a homogenizing valve with a "sharp edge" or "knife edge" passage head in order to achieve a 100% cell disruption rate with a single passage, at a dynamic pressure equal to or above 2000 bar.The "sharp edge" or "knife edge" profile of the passage head (4) is characterized by an inside diameter (9) of 10.9-14 mm and an outside diameter (10) of 11.9 mm-15 mm, and operates at a flow rate of 100-500 liters/hour and a dynamic pressure of more than 2000 bar.