A method for purifying bone-derived osteogenic proteins including a
demineralization process, a protein extraction process, a high molecular
weight ultrafiltration process, a low molecular weight ultrafiltration
process, and a recovery process. The high and low ultrafiltration
processes preferably select proteins having a nominal molecular weight
between approximately 8 kilodaltons and approximately 50 kilodaltons.
Processes of the present invention may be used to recover osteogenic
proteins from bone demineralization waste streams.