The invention relates to a method for growth of bacteria, such as Gram
negative bacteria and its corresponding supernatant. The bacteria are
cultivated without substantial release of endotoxins from the bacteria
into the surrounding medium. Thereby the need of purification steps
necessary to remove endotoxins are reduced or eliminated such as when the
bacteria are producing a product which later on should be administered to
a mammal, such as a human. The bacteria are also cultivated without
substantial formation of acetic acid. The level of the carbon/energy
source is controled during the cultivation and oxygen limitation is
avoided through regulation of the temperature. The method may be used for
cultivation of bacterial in fermenters.