A universal test apparatus for detecting tuberculosis (TB) in any of many different species of non-primate mammals is provided. The universal test apparatus includes a test site having a mycobacterial antigen cocktail comprising ESAT-6/CFP10 and 16 kDa/MPB83 polyfusion antigens, and a tracer having the ESAT-6/CFP10 polyfusion antigen and MPB83 antigen conjugated to latex or colloidal gold. The universal test apparatus is effective across genuses, families and orders of non-primate mammals.