The objective is to isolate and identify a gene relating to the regeneration ability of a plant, and to provide the gene and methods of use thereof. Isogenic lines to which culture characteristics of Konansou was introduced were produced by backcrossing. Culture cells were induced from this isogenic line and its parent variety. After confirming the regeneration ability of culture cells cultured for 3 to 6 months, isolation of a gene expressed in culture cells derived from Konansou and an isogenic line having a high regeneration ability, but not expressed in culture cells derived from Sasanishiki and Koshihikari was conducted using the differential display method. As a result, a regeneration-related gene was successfully cloned.