High frequency interfacing to biochemical membranes, such as supported bilayers and cell membranes, is carried out by supporting a biochemical membrane on a support surface while allowing access to the surface of the membrane through an opening in the support. A sharp tipped probe is positioned adjacent to the exposed surface of the membrane. The probe may have an inner core tip and a coaxial shield around the core tip that is electrically insulated therefrom. Radio frequency power is supplied to the probe to apply a localized radio frequency field to the membrane adjacent to the probe. Transport and binding events at the membrane are detected by changes in the field transmitted through the membrane and received by a receiving probe, or reflected from the membrane and received by the transmitting probe, and coupled therefrom to a detector for detection.