A sterilizable bio-reactor comprising a three-dimensional reactor culture
assembly substantially equivalent to a bone marrow micro environment, an
inert bio-compatible scaffolding material located on a base of the
reactor culture assembly to provide a micro environment identical to the
micro environment in human bone marrow, for cultivating stem cells in the
scaffolding, forming part of a sterilizable bio-reactor device, and a
process for the expansion of haemopoietic stem cells derived from Human
Umbilical Cord Blood for therapeutic use, comprising growing haemopoietic
stem cells in the bio-degradable non-toxic bio-compatible scaffold while
providing necessary nutrition and gasses cultivating the stem cells in
the scaffolding material to provide for the cell growth in the bio
reactor, and supplying mobilizing oxygen and carbon dioxide to maintain
gas tension for optimal expansion of the stem cells, and circulating
media containing micro and macro nutrients and growth factors to
establish gradients within the enclosure.