A puncture sealant supplying/removing apparatus includes a pressure-resistant bottle, an apparatus body on which the pressure-resistant bottle can be mounted, supply hose means which supplies or returns a puncture sealant accommodated in the pressure-resistant bottle to or from a tire, and a high pressure air source.The apparatus body comprises a venturi flow-path having a venturi portion, a first flow-path extending from the venturi portion into the pressure-resistant bottle, and a second flow-path extending from an inside of the pressure-resistant bottle and connected to one end of the supply hose means. The venturi flow-path is provided at its one end with an air intake which is connected to the high pressure air source, the venturi flow-path is provided at its other end with an opening/closing tool capable of opening and closing the other end.