The subject application concerns novel materials and methods for controlling non-mammalian pests. In a certain embodiments, the subject materials and methods for the control of coleopteran or lepidopteran pests are provided. The subject application also provides pesticidal proteins and compositions comprising pesticidal proteins that are derived from Beauvaria bassiana. These proteins have molecular weights that are greater than about (or greater than) 5000 daltons. In a preferred embodiment, the subject invention concerns plants cells, plant parts or plants to which the pesticidal proteins, or compositions thereof, have been applied.