A polymer based enzyme electrode useful for estimation for cholesterol is disclosed. The electrode has an ion-doped layer to increase the conductivity of the poly pyrrole layer. The ion doping is achieved by incorporating dodecyl benzene sulphonate (DBS) in the poly pyrrole film deposited by electrochemical deposition The enhanced conductivity due to ion doping helps in faster estimation of cholesterol having a measured response time of 30 seconds. The shelf life of the electrode is at least eight weeks without a mediator at room temperature.