A wireless treatment target positioning system for therapeutic radiation treatment includes a patient positioning system and a treatment target locating system. The treatment target locating system includes a room beam coordinate system and a localizing system. The room beam coordinate system includes devices for generating laser beams intersecting at a single point that is spatially coincident with an iso-center of the treatment system. The localizing system includes a movable reference object and a localizer for detecting and determining the position of the movable reference object. A method of positioning a treatment target to the iso-center of the treatment system includes a simulation process and real treatment positioning process. The simulation process includes positioning the treatment target to an iso-center of a simulation system and marking intersection points where laser beams intersect with an exterior of the patient's body. The real treatment positioning process includes determining the position of the intersection points marked on the exterior of the patient body, determining a treatment position for positioning the patient such that the treatment target is aligned with the iso-center of the real treatment system, and positioning the patient to the treatment position.