The present invention provides a method of hybridization, differential
methylation hybridization (DMH) for high throughput methylation analysis
of multiple CpG island loci. DMH utilizes nucleic acid probes prepared
from a cell sample to screen numerous CpG dinucleotide rich fragments
affixed on a screening array. Positive hybridization signals indicate the
presence of methylated sites. Methods of preparing the hybridization
probes and screening array are also provided.