An in-line night-day boresight with an adjustable wedge-prism assembly is disclosed. The adjustable wedge-prism assembly includes two opposing wedge prisms that are axially rotatable relative to each other. The assembly is arranged in the optical path between the night optics and the day optics. The adjustable wedge-prism assembly allows for compensation of image shift errors introduced by the night optics due to manufacturing errors in the night optics. This in turn allows for a cost-effective and easily aligned in-line night-day boresight.