Imaging layers, image recording media, and methods of preparation of each, are provided. One exemplary embodiment of the imaging layer, among others, includes a matrix, a radiation absorbing compound dissolved in the matrix, at least two activators substantially dissolved in the matrix, and a color former. The activators can include a primary activator having a higher acidity than a secondary activator. In addition, the primary activator has a lower solubility in the matrix than the secondary activator. The color former is substantially insoluble in the matrix at ambient conditions and is substantially uniformly distributed in the matrix.

 
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> Universal RT-coupled PCR method for the specific amplification of mRNA

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