The present invention provides an integrated analytical biochip and manufacturing method thereof, mainly by using a speedy and low-cost manufacturing process to integrate the Polymerase Chain Reaction ("PCR") reaction tank, Capillary Electrophoresis ("CE") channel and the fiber structure onto a single biochip, thus a DNA sample may be amplified via PCR on the biochip, and immediately driven into the CE channel for analysis in accordance with real-time online detection conducted through the fiber structure integrated on the biochip, such that a speedy and accurate process of biological sample detection and analysis can be achieved.