According to the invention, there is provided a hybrid maize plant, designated as 39F27, produced by crossing two Pioneer Hi-Bred International, Inc. proprietary inbred maize lines. This invention relates to the hybrid seed 39F27, the hybrid plant produced from the seed, and variants, mutants, and trivial modifications of hybrid 39F27. This invention also relates to methods for producing a maize plant containing in its genetic material one or more transgenes and to the transgenic maize plants produced by those methods. This invention further relates to methods for producing maize lines derived from hybrid maize line 39F27 and to the maize lines derived by the use of those methods.