A DNA-polypyrrole based biosensor and methods of using the biosensor for the rapid detection of Escherichia Coli and other microorganisms. The DNA-polypyrrole biosensor can be used to detect dangerous micoorganisms for monitoring water quality of a sample from a drinking water or food source. The biosensor can use genomic DNA extracted from natural environments in field settings for the rapid detection of microorganisms to provide an early warning of water contamination.

 
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