An enzymatically active reusable sponge or foam, capable of regeneration
with oximes, made of a polymer such as polyurethane is prepared for
detoxification of hazardous compounds such as organophosphorus and
organosulfur compounds. The foam or sponge contains a plurality of enzymes
including enzymes selected from acetylcholinesterase,
butyrylcholinesterase, triesterase, pseudocholinesterase, organophosphate
hydrase, phosphotriesterase, paraoxonase and organophosphorus and
organosulfur hydrolyzing enzymes. The sponge or foam may additionally
contain activated carbon and an enzyme reactivation compound. A kit can be
formed containing the sponge or foam and the compound for enzyme
reactivation. The enzymatically active foam or sponge may be prepared
using a two chamber device where enzymes and prepolymer are passed from
separate chambers into a static mixing stator and are subjected to low
shear mixing and extrusion to form by the sponge or foam.