The present invention provides for the production of homozygous primary
cells that carry a specific transgenic integration of interest on both
chromosomes by bypassing breeding. These cell lines can they be used for
the accelerated production of homozygous transgenic animals by somatic
cell nuclear transfer. The invention is thus useful in the production of
transgenic ungulate animals capable of producing desired
biopharmaceuticals in their milk at higher yield than a comparable
heterzygote. By combining the selection techniques of the current
invention with somatic cell nuclear transfer it can be applied to large
animals, where there is a strong need to shorten the time to
homozygosity.