An enclosure for handling hazardous material includes a sealable housing which is configured with a first air inlet connected to a bubble gauge and a second air inlet disposed inside the housing for air evacuation from the housing is connected to a pump for maintaining subatmospheric pressure in the enclosure and for drawing a stream of air inwardly through the first air inlet via the bubble gauge into the housing and out the second air inlet. A filter interposed in the stream of air leading to the second inlet. At least one glove port is formed in the housing and has a flexible relatively gas impermeable glove connected thereto. An air exchange port in the housing is operably connected to a sealed bellows which provides for changes in air volume within the enclosure which occurs from moving the glove within the enclosure under subatmospheric pressure.