An internal artificial vagina for collecting animal semen including a stiff support frame comprising a straight bar portion having at one end thereof a generally circular ring portion substantially perpendicular to the axis of the bar portion and an attachment means at the other end of the bar portion. An elastomeric tube open at both ends is adapted to slide within the frame ring portion with an end portion of the elastomeric tube stretched and pulled outwardly around the frame ring portion whereby the ring portion is retained within the folded portion of the elastomeric tube. The other end portion of the elastomeric tube is fixed to the attachment means of the frame. A replaceable semen collection bag is also provided in the form of an elongated generally cylindrical thin, flexible plastic member having a closed end and an open end. The collection bag is adapted to slide over the elastomeric tube with the open end being detachably connected to the elastomeric tube and the collection bag having a length substantially greater than the length of the elastomeric tube.