The invention relates to a modified phage display method for detecting and
identifying target and target binders. The modified methods involve
transforming host cells with two separate phages of a target or target
library and a target-binder library, and selecting to eliminate the
non-paired targets and binders. The invention also relates to antibodies
that bind to complexes of target and target binders; nucleic acids
encoding target and target binders peptides; and the use of sequence
information inherent in the targets and target binders for target
validation using in silico approaches. The invention further relates to
diagnostics and therapeutics employing the disclosed target and target
binder peptides or polynucletides and their use in small molecule drug
discovery; diagnostic and therapeutic applications directed to cancers
and similar disorders; and site directed assays for high throughput drug
screening.