Methods and apparatus for producing vaccine within a plurality of eggs are provided. Each of a plurality of eggs is illuminated with light from one or more LEDs. A detector is positioned adjacent each egg and detects light passing therethrough. Each egg is then identified as containing a live embryo or as being a non-live egg. Each egg that is determined not to contain a live embryo may be removed, either automatically or by hand. A seed virus is injected into each egg identified as containing a live embryo. After a predetermined period of incubation each live embryo is euthanized and amniotic fluid containing a vaccine produced as a result of the presence of a seed virus is harvested from each euthanized egg.