A cleaning mat system is designed for placement on a floor and for cleaning the undersides of shoes, feet, and other objects. The cleaning mat system includes a base with an upper surface and a lower surface interconnected by a perimeter edge. The perimeter edge includes a leading edge and an opposed trailing edge. The upper surface of the base includes upwardly extending teeth covering the majority of the upper surface, and the lower surface includes downwardly extending teeth on at least a portion of the lower surface adjacent the leading and trailing edges. A removable and disposable cleaning sheet has an upper face for cleaning and an opposed lower face. The cleaning sheet has a pair of spaced-apart side edge portions and a central portion. In use, the cleaning sheet is positioned on the base such that the central portion is adjacent the upper surface of the base and the side edge portions wrap around the leading and trailing edges to be trapped between the teeth and the floor.