The present invention relates to methods of using nucleotide sequences from the promoter region of at least one of seven S. cerevisiae genes whose expression is an indicator of the inhibition or modulation of the glucan synthase pathway in S. cerevisiae. This invention envisions using at least one target polynucleotide sequence, each target polynucleotide sequence being operably linked to the promoter region of one of the seven glucan synthase pathway reporter genes, to screen chemical libraries and natural products for compounds which can be used as antifungal agents for use against a variety of fungal pathogens. This invention also envisions using the methods of the invention to assay the efficacy of and/or specificity of antifungal agents, and/or to monitor the activity of the glucan synthase pathway.