The present invention relates to novel LRF-1 and LRF-2 proteins which are
related to the CRISP family and a protein called "Neutrophil Inhibitory
Factor (NIF)" isolated from the canine hookworm (Ancylostoma caninum)
that potently inhibits CD11/CD18-dependent neutrophil function. In
particular, isolated nucleic acid molecules are provided encoding the
human LRF-1 and LRF-2 proteins. LRF-1 and LRF-2 polypeptides are also
provided, as are vectors, host cells and recombinant methods for
producing the same. The invention further relates to screening methods
for identifying agonists and antagonists of LRF-1 or LRF-2 activity. Also
provided are diagnostic methods for detecting immune system or other
LRF-1- or LRF-2-related disorders and therapeutic methods for treating
such disorders.