Enzymatic digests of whey protein concentrates were prepared using animal,
bacterial and fungal proteases, and evaluated for antihypertensive activities.
The highest ACE-inhibitory activity was obtained with the purified peptide -1g
(f142-148) obtained by chemical synthesis, for which an IC50 value of
0.04 mg powder.ml-1 was found. The hydrolysates derived from BiPRO
whey protein isolate and -1g both gave higher antihypertensive activities
(IC50 values of 0.29 to 0.90 mg powder.ml-1) than the other
hydrolysates tested (IC50 values of 0.96 and 1.30 mg powder.ml-1).
The recovered hydrolysate can be used to treat hypertension in mammals such as
humans and domestic pets such as dogs and cats.
