A process for the production of inactivated Hepatitis A virus substantially free
of host cell contamination is described, the process comprising: a) culturing Hepatitis
A virus and harvesting a Hepatitis A preparation; b) treating said Hepatitis A
preparation with a protease; and thereafter c) separating intact virus from protease
digested material; d) inactivating said virus. Also described are vaccines comprising
the inactivated Hepatitis A virus, preferably in combination with strong adjuvants.