A tire puncture sealant comprises a latex of natural rubber, an emulsion of a tackifier and an antifreezing agent, wherein the tackifier is an aromatic modified terpene resin, and given that the total A+B+C of the solid content A of the natural rubber latex, the solid content B of the tackifier emulsion and the content C of the antifreezing agent is 100 weight %, the solid content A of the natural rubber latex is in a range of from 30 to 60 weight %, the solid content B of the tackifier emulsion is in a range of from 10 to 30 weight %, and the content C of the antifreezing agent is in a range of from 20 to 50 weight %.