A system, kit and process for the separation, recovery, purification and
identification of biomarkers such as peptides and the like from serum or
plasma. The process is to filter the sample through a UF filtration
device optionally having a vertical or substantial vertical ultrafilter
membrane, especially when in a single well configuration device. The
filtrate is recovered and if desired desalted using various
chromatography media such as reverse phase and ion exchange media. The
sample is then applied to a MALDI/TOF target or HPLC column and analyzed
in a mass spectrometer the presence, absence or variation of the
biomarkers. Higher recovery and concentration of these biomarkers is
achieved with the present invention.