The present invention relates to a method capable of detecting
proliferative bacteria in food and beverage, particularly acid or neutral
beverage at low cost within a short time, a detection kit which enables
simple measurement, and a technology for examination of harmful bacteria
in food and beverage with respect to a method for rapid determination of
proliferation properties of bacteria capable of producing guaiacol in
food and beverage.
There is disclosed a method for detecting bacteria capable of producing
guaiacol, which comprises culturing or incubating a culture solution
containing a specimen for a predetermined time in the presence of
vanillic acid, and measuring the produced guaiacol by qualitative or
quantitative analysis.
Also there is disclosed a kit for detection of bacteria capable of
producing guaiacol, comprising a container, and an acid or neutral
culture medium containing a vanillic acid substrate for production of
guaiacol, which is contained in the container and also sterilized and
sealed.
Furthermore there is disclosed a method for rapid determination of
proliferation properties of bacteria capable of producing guaiacol in
food and beverage, which comprises incubating a sporophyte of
spore-forming bacteria capable of producing guaiacol for a predetermined
time in the food and beverage in the presence of vanillic acid, and
measuring the produced guaiacol by qualitative or quantitative analysis.