The present invention provides a deproteinized natural rubber latex wherein coagulation
of a rubber component does not occur when the concentration of calcium ions (Ca2+)
is 0.01 mol/L or less and coagulation of the rubber component occurs when the concentration
of Ca2+ is 0.1 mol/L or more; a method of preparing the deproteinized
natural rubber latex, which comprises adding a protease and two or more surfactants
having different coagulation properties to calcium ions (Ca2+) to a
natural rubber latex and maturing the natural rubber latex; a rubber product using
the deproteinized natural rubber latex; and a proteolytic agent comprising a protease
and two or more surfactants. The deproteinized natural rubber latex is a latex
wherein rubber molecules are dispersed and stabilized by a surfactant, and also
have good film forming properties by means of the anode coagulation method and
is less likely to cause uneven thickness of the film and liquid dripping even when
a mold is dipped repeatedly in a latex on formation of a rubber film.