An electrophoretic apparatus comprising: a first electrolyte chamber containing
a first electrode; a second electrolyte chamber containing a second electrode;
a first sample chamber disposed between the first and second electrolyte chambers
and proximate to the first electrolyte chamber; a second sample chamber disposed
between the first sample chamber and the second electrolyte; three ion-permeable
barriers separating the first electrolyte chamber, the first sample chamber, the
second sample chamber, and the second electrolyte chamber, respectively, wherein
the ion-permeable barriers impede convective mixing of the contents in each of
the respective chambers; a first electrolyte reservoir and a second electrolyte
reservoir in fluid communication with the first and second electrolyte chambers,
respectively; a first sample reservoir and a second sample reservoir in fluid communication
with the first and second sample chambers, respectively; means adapted for communicating
a first electrolyte and a second electrolyte between the respective electrolyte
chambers and reservoirs; means adapted for communicating a first fluid and a second
fluid between the respective sample chambers and reservoirs, wherein at least one
of the first and second fluid contains at least a sample, wherein application of
an electric potential causes migration of at least one component through at least
one of the ion-permeable barriers.
