A method for characterizing fluorescent molecules or other particles in samples
comprising the steps of:
- a) monitoring fluctuating intensity of fluorescence emitted by the molecules
or other particles in at least one measurement volume of a non-uniform spatial
brightness profile by measuring numbers of photon counts in primary time intervals
by a single or more photon detectors,
- b) determining at least one distribution of numbers of photon counts,
{circumflex over (P)}(n), from the measured numbers of photon counts,
- c) determining physical quantities characteristic to said particles
by fitting the distribution of numbers of photon counts {circumflex over (P)}(n),
- wherein the fitting procedure involves calculation of a theoretical
distribution function of the number of photon counts P(n) through its generating
function, defined as
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