A one-pot glycosylation reaction is disclosed in which a mannosyl (Man) group
is
enzymatically transferred to an acceptor molecule. The starting glycoside is a
mannosyl 1-phosphate that is enzymatically converted to its GDP derivative via
UTP and a pyrophorylase. The formed GDP derivative is used in the enzyme-catalyzed
glycosyl transfer. That enzyme-catalyzed glycosyl transfer to an acceptor releases
GDP that is enzymatically converted to GTP for further conversion of mannosyl 1-phosphate
into its GDP derivative. Also disclosed are a recombinant 1,2-mannosyltransferase
that is enzymatically active, is dispersible in an aqueous reaction medium, and
free of the transmembrane portion of the native enzyme, as well as DNA encoding
that transferase, an expression vector containing exogenous DNA that encodes that
enzyme and E. coli cells containing that vector.
