The present invention relates to a process for separating nucleic acid molecules,
preferably open circular and supercoiled plasmid DNA and RNA molecules from each
other, comprising the steps of providing a solution comprising the molecules; adsorbing
the molecules to adsorbing groups on a carrier; and optionally washing the column
with a suitable solution. The present process is especially suitable for large-scale
isolation of supercoiled ccc DNA to be used in gene therapy.
