A method and apparatus for embedding cells that utilizes a flow-through embedding
technique maximizes the efficiency of extractions and decreases time for embedding
the cell fragments, minimizes cell loss, and automatically positions cell samples
at the position in which a microtome blade will section them. The apparatus includes
a cell flow pathway defined by an inflow tube for delivering cell fragments from
a cell sample to a sample port. The sample port is in fluid communication with
a tissue cassette having attached thereto a filter. The cell flow pathway is in
communication with a reagent flow pathway for delivering the reagents through the
sample port to the cassette. The apparatus is configured such that the application
of pressure directs the cell fragments from the cell sample through the cell flow
pathway, and effects delivery of the reagents through the reagent flow pathway.
The apparatus produces an embedded cell block having concentrated cells near the
plane of the block to be sectioned in a quick and efficient manner.
